The LC/GC The Column November issue is live. This issue features a review of alternative approaches to narrow standard calibration by Dr. Wolfgang Radke.
Molar mass determination by GPC/SEC requires column calibration. This is usually performed using narrowly distributed polymer standards of known molar mass. However, by this procedure true molar masses result only, if the chemical structure of the analyte matches the one of the standards used for calibration. As narrowly distributed standards of appropriate chemical structure are often not available, this instalment of Tips & Tricks describes alternative approaches to narrow standard calibration.