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T&T GPC/SEC: Aqueous GPC/SEC for Water-Soluble Macromolecules
Water-soluble macromolecules require water as a mobile phase, and method development therefore seems to be straightforward. At second glance, however, aqueous gel permeation…
Sprache

Veröffentlichungsnummer
20510
Publication Type
Tip&Trick
Quelle
The Column
Jahr
2020
Veröffentlicht
06.04.2020
Ausgabe
4
Autor
D. Held, W. Radke
T&T GPC/SEC: Beware of mismatch
Can I tell if a chromatogram's shoulder or side peak is part of my sample? Is it superior GPC/SEC resolution or artefact?
Sprache

Veröffentlichungsnummer
20272
Publication Type
Tip&Trick
Quelle
The Column
Jahr
2008
Veröffentlicht
29.05.2008
Ausgabe
4
Autor
T. Hofe
T&T GPC/SEC: Branching Analysis
Branching is one of the parameters chemists can adjust to produce polymer materials with optimized physical properties. Chromatography and advanced detection can help to characterize branched…
Sprache

Veröffentlichungsnummer
20444
Publication Type
Tip&Trick
Quelle
The Column
Jahr
2016
Veröffentlicht
07.06.2016
Ausgabe
6
Autor
D. Held, P. Montag, W. Radke
T&T GPC/SEC: Calibration Using Broad Standards
For polymeric materials where no narrowly distributed molar mass standards are available, reliable and true molar masses can be obtained using well characterized samples of broad molar mass…
Sprache

Veröffentlichungsnummer
20443
Publication Type
Tip&Trick
Quelle
The Column
Jahr
2015
Veröffentlicht
10.02.2015
Ausgabe
2
Autor
W. Radke, D. Held
T&T GPC/SEC: Column Issues with Light Scattering Detectors
The addition of a light scattering detector dramatically increases the capabilities of gel permeation chromatography/size-exclusion chromatography (GPC/SEC) analysis. However, the complexity of the…
Sprache

Veröffentlichungsnummer
20477
Publication Type
Tip&Trick
Quelle
The Column
Jahr
2019
Veröffentlicht
11.04.2019
Ausgabe
4
Autor
T. Hofe, M. Susewind
T&T GPC/SEC: Column Order in GPC/SEC
This instalment of Tips & Tricks will discuss the effect of column order on separation efficiency.
Sprache

Veröffentlichungsnummer
20528
Publication Type
Tip&Trick
Quelle
The Column
Jahr
2022
Veröffentlicht
24.08.2022
Ausgabe
4
Autor
W. Radke
T&T GPC/SEC: Copolymer Analysis in GPC/SEC_1: Compositional analysis by GPC/SEC with multiple detection
GPC/SEC analysis reveals a multitude of molar mass information about unknown samples which are chemically simple or homogenous. However, results for copolymers may be compromised by the fact that…
Sprache

Veröffentlichungsnummer
20339
Publication Type
Tip&Trick
Quelle
The Column
Jahr
2010
Veröffentlicht
08.06.2010
Ausgabe
6
Autor
P. Kilz
T&T GPC/SEC: Copolymer Analysis in GPC/SEC_2: Polymer Liquid Adsorption Chromatography
The CCD can be determined using several techniques including dual detection (see Tip&Trick copolymer analysis part 1). In this case GPC/SEC separation based on the size (hydrodynamic volume) is…
Sprache

Veröffentlichungsnummer
20340
Publication Type
Tip&Trick
Quelle
The Column
Jahr
2010
Veröffentlicht
20.08.2010
Ausgabe
8
Autor
P. Kilz
T&T GPC/SEC: Cumulative Distributions and Cumulative Match Calibration
Typically calibration in GPC/SEC is accomplished using narrowly distributed standards of known molar masses yielding relative molar masses for the analytes. If narrowly distributed standards having…
Sprache

Veröffentlichungsnummer
20512
Publication Type
Tip&Trick
Quelle
The Column
Jahr
2021
Veröffentlicht
19.01.2021
Ausgabe
1
Autor
D. Held, W. Radke
T&T GPC/SEC: Do's and Don'ts for Data Analysis_
GPC/SEC data processing requirements are fundamentally different from those of other liquild chromatographic techniques like for example HPLC. GPC/SEC users should follow some basics rules when the…
Sprache

Veröffentlichungsnummer
20386
Publication Type
Tip&Trick
Quelle
The Column
Jahr
2013
Veröffentlicht
21.03.2013
Ausgabe
2
Autor
D. Held