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T&T GPC/SEC: How does the Flow rate influence GPC/SEC results
Molar mass determination in GPC/SEC using a calibration routine with reference materials is based on the precise measurement of the elution volume of the sample. Neither the peak height, nor the peak…
Sprache

Veröffentlichungsnummer
20315
Publication Type
Tip&Trick
Quelle
The Column
Jahr
2009
Veröffentlicht
09.07.2009
Ausgabe
6
Autor
T. Hofe, D. Held
T&T GPC/SEC: How GPC/SEC Can Help to Reduce PET Plastic Waste
One of the major problems with plastics is recycling. Only a few materials can be recycled and the acceptance of recyclates is sometimes low. Gel permeation chromatography/size-exclusion…
Sprache

Veröffentlichungsnummer
20478
Publication Type
Tip&Trick
Quelle
The Column
Jahr
2019
Veröffentlicht
13.06.2019
Ausgabe
6
Autor
D. Held, P. Kilz
T&T GPC/SEC: How to Care for Your GPC/SEC Instrument
Columns are at the heart of a gel permeation/size-exclusion chromatography (GPC/SEC) system. Software is used to evaluate the data, to understand and present the results, and to act as an interface…
Sprache

Veröffentlichungsnummer
20432
Publication Type
Tip&Trick
Quelle
The Column
Jahr
2015
Veröffentlicht
29.10.2015
Ausgabe
10
Autor
D. Held
T&T GPC/SEC: How to Choose a Static Light Scattering Technique for Molar Mass Determination
Light scattering is one of the few absolute methods for the characterization of macromolecules and biopolymers. On-line light scattering detectors are used in GPC/SEC systems to measure molar masses,…
Sprache

Veröffentlichungsnummer
20297
Publication Type
Tip&Trick
Quelle
The Column
Jahr
2009
Veröffentlicht
29.04.2009
Ausgabe
4
Autor
D. Held, P. Kilz
T&T GPC/SEC: How to Determine dn/dc Values
The refractive index increment (dn/dc) is an essential parameter when analyzing light-scattering (LS) data, independent of the LS method applied. This instalment of Tips & Tricks discusses the…
Sprache

Veröffentlichungsnummer
20440
Publication Type
Tip&Trick
Quelle
The Column
Jahr
2015
Veröffentlicht
12.06.2015
Ausgabe
4
Autor
D. Held
T&T GPC/SEC: How to expand the lifetime of columns
Unlike HPLC columns, GPC/SEC columns are made of crosslinked polymeric materials with a permanent porosity. Due to the permanent porosity GPC/SEC columns are pressure sensitive and need more care than…
Sprache

Veröffentlichungsnummer
20317
Publication Type
Tip&Trick
Quelle
The Column
Jahr
2009
Veröffentlicht
08.12.2009
Ausgabe
12
Autor
G. Reinhold
T&T GPC/SEC: How to find the ideal stationary GPC/SEC separation phase?
What are the driving forces for separation in GPC/SEC and how do they influence method development?
Sprache

Veröffentlichungsnummer
20262
Publication Type
Tip&Trick
Quelle
The Column
Jahr
2007
Veröffentlicht
31.12.2007
Ausgabe
12
Autor
T. Hofe, G. Reinhold
T&T GPC/SEC: How to Install GPC/SEC Columns
For proper column installation it is important to be familiar with column characteristics such as solvent compatibility, maximum fl ow rate, and maximum operating temperature. This article focuses on…
Sprache

Veröffentlichungsnummer
20413
Publication Type
Tip&Trick
Quelle
The Column
Jahr
2014
Veröffentlicht
07.02.2014
Ausgabe
2
Autor
D. Held
T&T GPC/SEC: How to Test GPC/SEC Columns
Gel permeation chromatography/size-exclusion chromatography (GPC/SEC) columns are at the heart of a GPC/SEC system. Even when advanced detection, such as on-line light scattering, viscometry, or mass…
Sprache

Veröffentlichungsnummer
20420
Publication Type
Tip&Trick
Quelle
The Column
Jahr
2014
Veröffentlicht
12.08.2014
Ausgabe
8
Autor
F. Gores, D. Held
T&T GPC/SEC: How to treat your RI Detector
The refractive index (RI) detector is the most common detector in gel permeation chromatography/size-exclusion chromatography (GPC/SEC). The advantage of this universal detector is that it detects…
Sprache

Veröffentlichungsnummer
20433
Publication Type
Tip&Trick
Quelle
The Column
Jahr
2017
Veröffentlicht
05.12.2017
Ausgabe
12
Autor
D. Held