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GPC/SEC Tips&Tricks


Developed by PSS for LC/GC: Practical tips and advice for GPC/SEC users.

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Publications

T&T GPC/SEC: _Result Uncertainty - How reliable are Results?
GPC/SEC results and their validity are crucial in many applications, e.g. as part of a QC/QA test for a product release, the registration and accreditation of polymeric products or formulations with...
Language
Publication Number
20382
Publication Type
Tip&Trick
Source
The Column
Year
2012
Publication date
12.06.2012
Issue
6
Author
D. Held, P. Kilz
It's Magic: The PSS Column Concept
Goal for method development in GPC/SEC/GFC is the selection of the best solvent, sometimes plus salt or modifier, and the optimum stationary phase material, so that interaction cannot occur. A...
Language
Publication Number
20379
Publication Type
Poster
Source
PSS Product Information
Year
2012
Publication date
24.05.2012
Author
D. Held
T&T GPC/SEC: _What is the best detector sequence?_
The information and results that are available from detector combinations have been the subject of various Tips&Tricks over the years.This Tip&Trick discusses the general impact of detector...
Language
Publication Number
20384
Publication Type
Tip&Trick
Source
The Column
Year
2012
Publication date
24.05.2012
Author
D. Held, P. Kilz
T&T GPC/SEC: _How to get a stable Baseline
A stable baseline is a prerequisite for precise, accurate, and reproducible GPC/SEC analysis, especially for samples with a broad molar mass distribution. Baseline problems can include drift, wander...
Language
Publication Number
20381
Publication Type
Tip&Trick
Source
The Column
Year
2012
Publication date
11.05.2012
Issue
4
Author
D. Held
Food Analysis Application: High resolution Saccharide and Polysaccharide Analysis using Small Particles
The PSS SUPREMA column with a reduced particle size of 5 µm offers a significantimprovement in performance compared to traditional 10 µm materials and providesoutstanding additional resolution,...
Language
Publication Number
20403
Publication Type
Notes
Source
PSS Application
Year
2012
Publication date
11.05.2012
Author
G. Reinhold
Chemical Manufacturing Application: Absolute molar masses for phenol formaldehyderesins with GPC/SEC-ESI-MS
Hyphenation of an electrospray ionization-mass spectrometer (ESI-MS) with a GPC/SEC-System allows the determination of absolute molar masses for low molar mass samples or samples with an unknown...
Language
Publication Number
20404
Publication Type
Notes
Source
PSS Application
Year
2012
Publication date
11.05.2012
Author
D. Held, F. Gores
Biopharm Application: GPC/SEC-MALLS Study of Protein Denaturation
GPC/SEC-MALLS is a powerful tool to detect and follow the transition of protein structures. GPC/SEC allows the separation of the associates (dimer, trimer, etc.). Absolute molar masses can be detected...
Language
Publication Number
20405
Publication Type
Notes
Source
PSS Application
Year
2012
Publication date
11.05.2012
Author
T. Hofe
Food Analysis Application: Molecular weight determination of Gelatin
Reproducible and easy-to-use GPC/SEC analysis for all types of Gelatins can be done using a SECcurity GPC system and PROTEEMA columns. Exact sample preparation, following a strict protocol, and the...
Language
Publication Number
20406
Publication Type
Notes
Source
PSS Application
Year
2012
Publication date
11.05.2012
Author
D. Held, G. Reinhold
Medical Analysis Application: GPC/SEC-MALLS analysis of Hyaluronic Acid
Hyaluronic acid is a polymer of disaccharides and can have up to 25,000 disaccharide repeat units. The molar mass range is from 5000 Da to 20 000 000 Da. Hyaluronic acid is mainly used in medical and...
Language
Publication Number
20407
Publication Type
Notes
Source
PSS Application
Year
2012
Publication date
11.05.2012
Author
F. Gores, G. Reinhold
T&T GPC/SEC: Method Development_
The information and results that are available from detector combinations have been the subject of various Tips&Tricks over the years. Undiscussed up to know was the general impact of detector...
Language
Publication Number
20385
Publication Type
Tip&Trick
Source
The Column
Year
2012
Publication date
25.04.2012
Issue
12
Author
D. Held
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