Tips&Tricks has been developed by PSS for LC/GC Europe. Since February 2007 it is published bimonthly in LCGC Europe's The Column.
These Tips&Tricks are designed to support GPC/SEC users of in their daily work providing comprehensive overviews on different aspects of this powerful technique.
Many readers collect these publications and approached us for a collection. This resulted in 5 e-books, each book based on 5-8 different Tips & Tricks publications updated with the latest information, new examples and figures.
Please feel free to contact us to receive a pdf. If you miss a topic and want us to elaborate a topic please propose a topic or submit a problem.
A Few Things Chromatograms Can (and Can't) Tell
Cumulative Distributions and Cumulative Match Calibration
Separation Range and Resolution
Silica Versus Polymer‑Based Columns
Aqueous GPC/SEC for Water-Soluble Macromolecules
Particle Size and the Effect of Mixing Columns of Different Particle Size
Alternatives to Narrow Standard Calibration in GPC/SEC
System Peaks or Ghost Peaks in GPC/SEC
Systematic errors in GPC/SEC/GFC
Column Issues with Light Scattering Detectors
"Green" GPC/SEC and Solvent Alternatives
How GPC/SEC Can Help to Reduce PET Plastic Waste
High Temperature GPC versus ambient GPC
How Do Calibration Curves Influence GPC/SEC Results?
What you need to know to allow for efficient GPC/SEC Troubleshooting
Aqueous GPC/SEC: Influence of Salt and pH
What are differences between GPC, SEC and GFC and how to get started?
Quantify and get more than Molar Mass Averages
New and Old Approaches to Handle Unknown dn/dc in GPC/SEC-LS
Protein Analysis with Size-Exclusion Chromatography
How to Treat Your RI Detector
Product Registration and REACH
Upside Down - Inverse GPC for Pore Size Distributions
GPC/SEC for Membrane Analysis
Flow Marker - An Easy Concept to Increase Reproducibility
How Broad is Broad for Macromolecules?
GPC/SEC-Light Scattering: Garbage In, Garbage out
Calibration Using Broad Standards
How to Care for Your GPC/SEC Instrument
How to Determine dn/dc Values
Finding the Right Standards
Answering Common Questions about GPC/SEC Columns
Strategies to Save Solvent
How to Install GPC/SEC Columns
Sample Recovery and More
Mobile Phase Considerations
From a Chromatogram to the Molar Mass Distribution
The Art of Analyzing High Molar Mass Samples
How to test GPC/SEC Columns
GPC/SEC do's and don'ts for data analysis
Evaporative Light Scattering Detection in GPC/SEC
GPC/SEC Method Optimization
Select the Right Columns for Your Molar Mass Range
Analysis and Calibration for Low Molar Mass Macromolecules
An Alternative to Calibration with Narrow Standards
GPC/SEC-Viscometry – a Versatile Tool
What is the best detector sequence?
How to get a stable Baseline
GPC/SEC Method Development
Result Uncertainty - How reliable are Results?
Increase Resolution and Separation Range
Improving the Reliability of GPC/SEC Light Scattering and Viscometry Results
Mass spectrometry as a new solution
Is this real? Unexpected Peak Shapes
GPC/SEC columns - Dos and Don'ts
How to Validate a GPC/SEC System
Copolymer Analysis in GPC/SEC 3: 2-dimensional Chromatography
UGPC? Making GPC/SEC faster
Peak Identification in GPC/SEC
Copolymer Analysis in GPC/SEC 1: Compositional analysis by GPC/SEC with multiple detection
Copolymer Analysis in GPC/SEC 2: Polymer Liquid Adsorption Chromatography
Do’s and Don’ts in GPC/SEC-Light Scattering
How to expand the lifetime of columns
Choose a Static Light-scattering Technique
How does the Flow-rate Influence GPC/SEC Results
Temperature Effects in GPC/SEC
Accuracy and precision in GPC/SEC
Understanding positive and negative detector signals
BEWARE of Mismatch
A new development in high temperature (HT) GPC/SEC
How do I calibrate a GPC/SEC system?
What are the applications for (polymer) reference standards?
How do I find the optimum sample concentration and injection volume for my GPC/SEC measurements?
The importance of molar mass distributions
A look at the importance of molar mass averages.
How to find the ideal stationary GPC/SEC separation phase?