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Specifications PROTEEMA columns

Applicability Natural and synthetic Proteins, Peptides, Enzymes, Gelatins/Collagens
Eluents Water, water with salt/buffer; pH: 2 -9
Material Special modified silica
Maximum Pressure150 - 200 bar (2180 - 2900 psi), depending on porosity
Maximum Temperature70° C
Maximum Flow Rate(8 mm I.D.) 3 ml/min
Particle Size 3 µm, 5 µm
Molecular Weight Range 100 to 7 500 000 Da



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Standard Columns (others on request)

Separation Range [Da]*   Single Porosity Columns
100 - 150 000   100 Å
1 000 - 1 200 000   300 Å
10 000 - 7 500 000   1 000 Å

* based on protein molecular weight, for other water soluble macromolecules
(e.g. pullulane) separation range ends at 50 000, 400 000, and 2 500 000 Da.

 

Part numbers depend on particle size and dimension

Columns are available as Analytical or Micro Columns or in (metall-free) PEEK hardware.

 

Separation Range

Calibration Curves

Eluent: aqueous buffer
Calibrants: Pullulan

 

 

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