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Specifications SUPREMA columns

Applicability Neutral and anionic polymers, (PEO, PEG, Pullulan, Dextran, Poly(acrylamide), Hyaluronic acid, Poly(acrylic acid), Carboxymethylcellulose, etc.)
Eluents water (with salts/buffers, MeOH, ACN) pH: 1.5 – 13
Material Polyhydroxymethacrylate copolymer network
Maximum Pressure 50 - 80 bar (725 - 1160 psi), depending on porosity
Maximum Temperature 80° C
Maximum Flow Rate 2 ml/min (I.D.: 8 mm)
Particle Size 3, 5 and 10 µm
Molecular Weight Range100 to > 30 000 000 Da


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Standard Columns (others on request)

Separation Range [Da]   Preconfigured Column Sets
100 - 100 000   SUPREMA Combination low (P/N 206-0001)
100 - 1 000 000   SUPREMA Combination medium (P/N 206-0002)
100 - 3 000 000   SUPREMA Combination high (P/N 206-0003)
100 - 30 000 000   SUPREMA Combination ultrahigh (P/N 206-0004)
     
Separation Range [Da]   Single Porosity/Linear Columns
100 - 30 000   30 Å
100 - 100 000   100 Å
100 - 1 000 000   1 000 Å
1 000 - 3 000 000   3 000 Å
1 000 - 10 000 000   10 000 Å
5 000 - 30 000 000   30 000 Å
100 - 100 000   Linear S
1 000 - 1 000 000   Linear M
5 000 - 3 000 000   Linear XL
100 - 30 000 000   Ultrahigh
     

Part numbers depend on particle size and dimension

Columns are available as Analytical, Preparative, HighSpeed, or Micro Columns.

Separation Range

Calibration Curves: single porosity columns

Eluent: aqueous buffer
Calibrants: Pullulan

Calibration Curves: linear columns

Eluent: aqueous buffer
Calibrants: Pullulan

 

 

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