The use of smaller particles in liquid chromatography has the general advantage that higher resolution is achieved. An efficient separation can be achieved in a short time if small particle separation materials are used in columns with smaller dimensions (micro columns) at flow rates in the range of 0.5 - 0.7 mL/min.
Typical column dimensions for micro columns are 150 to 250 mm length and 4.6 mm internal diameter.
PSS micro columns are supplied in the dimensions of 4.6x250 mm and are available in various porosities for the following materials:
- for nonpolar and medium polar organic solvents: PSS SDV 3 μm
- for natural and synthetic proteins and peptides in aqueous solvents: PSS PROTEEMA 3 μm
- for neutral and anionic polymers in aqueous solvents: PSS SUPREMA 5 μm
- for fluorinated organic solvents: PSS PFG 5 μm
Other materials can be delivered on request.
Typically these conditions require instrumentation with small cell volumes and low overall dead volume. With the PSS µSECcurity² GPC system introducing an μRI with smallest cell volume (1.7 μL) a specifically designed solution supporting all micro columns is available. Existing PSS (Bio)SECcurity² GPC systems (as well as Agilent 1260/1290-Systems) can be seamlessly retrofitted at any time.
µRI detection cells allow for less band broadening and higher resolution
Practical strategies in GPC/SEC to increase the resolution.