GPC/SEC Tips&Tricks
Tips&Tricks has been developed by PSS for LC/GC Europe. Since February 2007 it is published bimonthly in LCGC Europe's The Column.
These Tips&Tricks are designed to support GPC/SEC users of in their daily work providing comprehensive overviews on different aspects of this powerful technique.
Many readers collect these publications and approached us for a collection. This resulted in 5 e-books, each book based on 5-8 different Tips & Tricks publications updated with the latest information, new examples and figures.
Please feel free to contact us to receive a pdf. If you miss a topic and want us to elaborate a topic please propose a topic or submit a problem.
Latest publications
The Column 01 / 2023
A look at molar mass averages and how they are affected by SEC baseline and integration settings?
Archive
2022
The Column 11 / 2022
Treating Your GPC/SEC System Properly
The Column 07 / 2022
Column Order in GPC/SEC
The Column 04 / 2022
Trouble Analyzing PEG's?
The Column 01 / 2022
A Few Things Molar Mass Distributions Can (and Can’t) Tell
2021
The Column 01 / 2021
Cumulative Distributions and Cumulative Match Calibration
The Column 04 / 2021
Separation Range and Resolution
The Column 08 / 2021
A Few Things Chromatograms Can (and Can't) Tell
The Column 11 / 2021
Influence of Sample Dispersity on the GPC/SEC Separation of Multi-Component Systems
2020
The Column 1 / 2020
Silica Versus Polymer‑Based Columns
The Column 4 / 2020
Aqueous GPC/SEC for Water-Soluble Macromolecules
The Column 8 / 2020
Particle Size and the Effect of Mixing Columns of Different Particle Size
The Column 11 / 2020
Alternatives to Narrow Standard Calibration in GPC/SEC
2019
The Column 2 / 2019
System Peaks or Ghost Peaks in GPC/SEC
The Column 10 / 2019
Systematic errors in GPC/SEC/GFC
The Column 4 /2019
Column Issues with Light Scattering Detectors
The Column 12 / 2019
"Green" GPC/SEC and Solvent Alternatives
The Column 6 / 2019
How GPC/SEC Can Help to Reduce PET Plastic Waste
The Column 8 / 2019
High Temperature GPC versus ambient GPC
2018
The Column 4 / 2018
How Do Calibration Curves Influence GPC/SEC Results?
The Column 12 / 2018
What you need to know to allow for efficient GPC/SEC Troubleshooting
The Column 6 / 2018
Aqueous GPC/SEC: Influence of Salt and pH
The Column 8 / 2018
UV-vis Detection
The Column 10 / 2018
What are differences between GPC, SEC and GFC and how to get started?
2017
The Column 2 / 2017
Quantify and get more than Molar Mass Averages
The Column 10 / 2017
New and Old Approaches to Handle Unknown dn/dc in GPC/SEC-LS
The Column 4 / 2017
Protein Analysis with Size-Exclusion Chromatography
The Column 12 / 2017
How to Treat Your RI Detector
The Column 6 / 2017
Product Registration and REACH
The Column 8 / 2017
Inter-Detector Delay
2016
The Column 2 / 2016
Upside Down - Inverse GPC for Pore Size Distributions
The Column 10 / 2016
GPC/SEC for Membrane Analysis
The Column 4 / 2016
Flow Marker - An Easy Concept to Increase Reproducibility
The Column 12 / 2016
How Broad is Broad for Macromolecules?
The Column 6 / 2016
Branching Analysis
The Column 8 / 2016
GPC/SEC-Light Scattering: Garbage In, Garbage out
2015
The Column 2 / 2015
Calibration Using Broad Standards
The Column 10 / 2015
How to Care for Your GPC/SEC Instrument
The Column 4 / 2015
How to Determine dn/dc Values
The Column 12 / 2015
Finding the Right Standards
The Column 6 / 2015
Answering Common Questions about GPC/SEC Columns
The Column 8 / 2015
Strategies to Save Solvent
2014
The Column 2 / 2014
How to Install GPC/SEC Columns
The Column 10 / 2014
Sample Recovery and More
The Column 4 / 2014
Mobile Phase Considerations
The Column 12 / 2014
From a Chromatogram to the Molar Mass Distribution
The Column 6 / 2014
The Art of Analyzing High Molar Mass Samples
The Column 8 / 2014
How to test GPC/SEC Columns
2013
The Column 2 / 2013
GPC/SEC do's and don'ts for data analysis
The Column 10 / 2013
Evaporative Light Scattering Detection in GPC/SEC
The Column 4 / 2013
GPC/SEC Method Optimization
The Column 12 / 2013
Select the Right Columns for Your Molar Mass Range
The Column 6 / 2013
Analysis and Calibration for Low Molar Mass Macromolecules
The Column 8 / 2013
An Alternative to Calibration with Narrow Standards
2012
The Column 2 / 2012
GPC/SEC-Viscometry – a Versatile Tool
The Column 10 / 2012
What is the best detector sequence?
The Column 4 / 2012
How to get a stable Baseline
The Column 12 / 2012
GPC/SEC Method Development
The Column 6 / 2012
Result Uncertainty - How reliable are Results?
The Column 8 / 2012
Faster Results
2011
The Column 2 / 2011
Increase Resolution and Separation Range
The Column 10 / 2011
Improving the Reliability of GPC/SEC Light Scattering and Viscometry Results
The Column 4 / 2011
Mass spectrometry as a new solution
The Column 12 / 2011
Is this real? Unexpected Peak Shapes
The Column 7 / 2011
GPC/SEC columns - Dos and Don'ts
The Column 8 / 2011
Sample preparation
2010
The Column 3 / 2010
How to Validate a GPC/SEC System
The Column 10 / 2010
Copolymer Analysis in GPC/SEC 3: 2-dimensional Chromatography
The Column 4 / 2010
UGPC? Making GPC/SEC faster
The Column 12 / 2010
Peak Identification in GPC/SEC
The Column 6 / 2010
Copolymer Analysis in GPC/SEC 1: Compositional analysis by GPC/SEC with multiple detection
The Column 8 / 2010
Copolymer Analysis in GPC/SEC 2: Polymer Liquid Adsorption Chromatography
2009
The Column 02 / 2009
Do’s and Don’ts in GPC/SEC-Light Scattering
The Column 12 / 2009
How to expand the lifetime of columns
The Column 4 / 2009
Choose a Static Light-scattering Technique
The Column 6 / 2009
How does the Flow-rate Influence GPC/SEC Results
The Column 8 / 2009
Temperature Effects in GPC/SEC
2008
The Column 2 / 2008
Accuracy and precision in GPC/SEC
The Column 10 / 2008
Understanding positive and negative detector signals
The Column 4 / 2008
BEWARE of Mismatch
The Column 12 / 2008
A new development in high temperature (HT) GPC/SEC
The Column 6 / 2008
How do I calibrate a GPC/SEC system?
The Column 8 / 2008
What are the applications for (polymer) reference standards?
2007
The Column 2 / 2007
How do I find the optimum sample concentration and injection volume for my GPC/SEC measurements?
The Column 8 / 2007
The importance of molar mass distributions
The Column 10 / 2007
A look at the importance of molar mass averages.
The Column 12 / 2007
How to find the ideal stationary GPC/SEC separation phase?