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Learn more about macromolecules, GPC/SEC and 2D.

GPC/SEC Tips&Tricks


Developed by PSS for LC/GC: Practical tips and advice for GPC/SEC users.

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PSS Publication Database

Publications

T&T GPC/SEC: Inter-Detector Delay
Combinations of detectors are often used in gel permeation chromatography/size-exclusion chromatography (GPC/SEC) to measure absolute molar masses or chemical compositions as a function of elution…
Language
Publication Number
20441
Publication Type
Tip&Trick
Source
The Column
Year
2017
Publication date
10.08.2017
Issue
8
Author
D. Held, W. Radke
T&T GPC/SEC: Product Registration and REACH
Molecular mass is one of the central parameters required for product registration. Compared to low molar mass substances, the molar mass determination of macromolecular products is more diffi cult…
Language
Publication Number
20436
Publication Type
Tip&Trick
Source
The Column
Year
2017
Publication date
20.06.2017
Issue
6
Author
D. Held
PSS e-Book Copolymer Analysis
In this e-book: - Compositional Analysis by GPC/SEC With Multiple Detection - Polymer Liquid Adsorption Chromatography - Two-Dimensional Chromatography of Copolymers - Comonomer Identification:…
Language
Publication Number
20538
Publication Type
e-book
Source
e-book
Year
2017
Publication date
16.06.2017
Issue
1
Analysis of a Graft Copolymer using Dual Detection and Interaction Chromatography
Characterization of complex (Co)polymers with multiple distributions require more than size-based separations (GPC/SEC). While multidetection GPC/SEC can provide interesting details, separations based…
Language
Publication Number
20471
Publication Type
Notes
Source
PSS Application
Year
2017
Publication date
08.06.2017
Author
M. Adler, R. Leinweber, J. Preis, W. Radke
GPC/SEC Tutorial #4: Choosing the Column
I have completely new samples and need to identify the best columns to analyze them - what are the correct column selection criteria for my analyses?
Language
Publication Number
20519
Publication Type
Webinar/Webcast
Source
PSS Webinar
Year
2017
Publication date
01.06.2017
Author
D. Held
T&T GPC/SEC: Protein Analysis with Size-Exclusion Chromatography
Gel permeation chromatography/size-exclusion chromatography (GPC/SEC) is the standard method to separate samples by molecular size. In protein analysis, size-exclusion chromatography is either applied…
Language
Publication Number
20437
Publication Type
Tip&Trick
Source
The Column
Year
2017
Publication date
16.05.2017
Issue
4
Author
D. Held, T. Hofe
GPC/SEC Tutorial #3: Unexpected Peak Shapes
Do you find weird peak shapes in your chromatograms? Not sure if they are real or artifacts? In this tutorial we'll discuss peak shapes and learn how to verify if a GPC / SEC chromatogram is realistic…
Language
Publication Number
20518
Publication Type
Webinar/Webcast
Source
PSS Webinar
Year
2017
Publication date
21.03.2017
Author
D. Held
T&T GPC/SEC: Quantify and Get More Than Molar Mass Averages
Gel permeation chromatography/size-exclusion chromatography (GPC/SEC) is used to determine molar mass averages and the complete molar mass distribution with just one injection. This is possible…
Language
Publication Number
20435
Publication Type
Tip&Trick
Source
The Column
Year
2017
Publication date
23.02.2017
Issue
2
Author
D. Held
T&T GPC/SEC: Polydispersity - How Broad is Broad for Macromolecules?
The vast majority of macromolecules exhibit a molar mass distribution that is often described by the polydispersity index (PDI). This Tips & Tricks instalment offers practical advice to consider when…
Language
Publication Number
20442
Publication Type
Tip&Trick
Source
The Column
Year
2016
Publication date
22.12.2016
Issue
12
Author
D. Held, W. Radke
SEC-Protein analysis
GPC/SEC is a powerful method to check protein purity. In combination with light scattering it is possible to determine molar masses and sizes. This webinar discusses the latest developments and new…
Language
Publication Number
20457
Publication Type
Webinar/Webcast
Source
PSS Webinar
Year
2016
Publication date
15.12.2016
Issue
4
Author
T. Hofe, G. Reinhold
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