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GPC/SEC Tips&Tricks


Developed by PSS for LC/GC: Practical tips and advice for GPC/SEC users.

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PSS Publication Database

Publications

T&T GPC/SEC: How to Choose a Static Light Scattering Technique for Molar Mass Determination
Light scattering is one of the few absolute methods for the characterization of macromolecules and biopolymers. On-line light scattering detectors are used in GPC/SEC systems to measure molar masses,…
Language
Publication Number
20297
Publication Type
Tip&Trick
Source
The Column
Year
2009
Publication date
29.04.2009
Issue
4
Author
D. Held, P. Kilz
T&T GPC/SEC: How to Determine dn/dc Values
The refractive index increment (dn/dc) is an essential parameter when analyzing light-scattering (LS) data, independent of the LS method applied. This instalment of Tips & Tricks discusses the…
Language
Publication Number
20440
Publication Type
Tip&Trick
Source
The Column
Year
2015
Publication date
12.06.2015
Issue
4
Author
D. Held
T&T GPC/SEC: How to expand the lifetime of columns
Unlike HPLC columns, GPC/SEC columns are made of crosslinked polymeric materials with a permanent porosity. Due to the permanent porosity GPC/SEC columns are pressure sensitive and need more care than…
Language
Publication Number
20317
Publication Type
Tip&Trick
Source
The Column
Year
2009
Publication date
08.12.2009
Issue
12
Author
G. Reinhold
T&T GPC/SEC: How to find the ideal stationary GPC/SEC separation phase?
What are the driving forces for separation in GPC/SEC and how do they influence method development?
Language
Publication Number
20262
Publication Type
Tip&Trick
Source
The Column
Year
2007
Publication date
31.12.2007
Issue
12
Author
T. Hofe, G. Reinhold
T&T GPC/SEC: How to Install GPC/SEC Columns
For proper column installation it is important to be familiar with column characteristics such as solvent compatibility, maximum fl ow rate, and maximum operating temperature. This article focuses on…
Language
Publication Number
20413
Publication Type
Tip&Trick
Source
The Column
Year
2014
Publication date
07.02.2014
Issue
2
Author
D. Held
T&T GPC/SEC: How to Test GPC/SEC Columns
Gel permeation chromatography/size-exclusion chromatography (GPC/SEC) columns are at the heart of a GPC/SEC system. Even when advanced detection, such as on-line light scattering, viscometry, or mass…
Language
Publication Number
20420
Publication Type
Tip&Trick
Source
The Column
Year
2014
Publication date
12.08.2014
Issue
8
Author
F. Gores, D. Held
T&T GPC/SEC: How to treat your RI Detector
The refractive index (RI) detector is the most common detector in gel permeation chromatography/size-exclusion chromatography (GPC/SEC). The advantage of this universal detector is that it detects…
Language
Publication Number
20433
Publication Type
Tip&Trick
Source
The Column
Year
2017
Publication date
05.12.2017
Issue
12
Author
D. Held
T&T GPC/SEC: How to validate a GPC/SEC system
Validation is not an easy task. It is an ongoing complex process that evaluates the entire, sample specific process, products, and analytical methods and consists of four qualification phases: DQ…
Language
Publication Number
20318
Publication Type
Tip&Trick
Source
The Column
Year
2010
Publication date
05.03.2010
Issue
3
Author
D. Held, C. Trimmer
T&T GPC/SEC: Inter-Detector Delay
Combinations of detectors are often used in gel permeation chromatography/size-exclusion chromatography (GPC/SEC) to measure absolute molar masses or chemical compositions as a function of elution…
Language
Publication Number
20441
Publication Type
Tip&Trick
Source
The Column
Year
2017
Publication date
10.08.2017
Issue
8
Author
D. Held, W. Radke
T&T GPC/SEC: Method Development_
The information and results that are available from detector combinations have been the subject of various Tips&Tricks over the years. Undiscussed up to know was the general impact of detector…
Language
Publication Number
20385
Publication Type
Tip&Trick
Source
The Column
Year
2012
Publication date
25.04.2012
Issue
12
Author
D. Held
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