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T&T GPC/SEC: Aqueous GPC/SEC for Water-Soluble Macromolecules
Water-soluble macromolecules require water as a mobile phase, and method development therefore seems to be straightforward. At second glance, however, aqueous gel permeation…
Language
Publication Number
20510
Publication Type
Tip&Trick
Source
The Column
Year
2020
Publication date
06.04.2020
Issue
4
Author
D. Held, W. Radke
T&T GPC/SEC: Beware of mismatch
Can I tell if a chromatogram's shoulder or side peak is part of my sample? Is it superior GPC/SEC resolution or artefact?
Language
Publication Number
20272
Publication Type
Tip&Trick
Source
The Column
Year
2008
Publication date
29.05.2008
Issue
4
Author
T. Hofe
T&T GPC/SEC: Branching Analysis
Branching is one of the parameters chemists can adjust to produce polymer materials with optimized physical properties. Chromatography and advanced detection can help to characterize branched…
Language
Publication Number
20444
Publication Type
Tip&Trick
Source
The Column
Year
2016
Publication date
07.06.2016
Issue
6
Author
D. Held, P. Montag, W. Radke
T&T GPC/SEC: Calibration Using Broad Standards
For polymeric materials where no narrowly distributed molar mass standards are available, reliable and true molar masses can be obtained using well characterized samples of broad molar mass…
Language
Publication Number
20443
Publication Type
Tip&Trick
Source
The Column
Year
2015
Publication date
10.02.2015
Issue
2
Author
W. Radke, D. Held
T&T GPC/SEC: Column Issues with Light Scattering Detectors
The addition of a light scattering detector dramatically increases the capabilities of gel permeation chromatography/size-exclusion chromatography (GPC/SEC) analysis. However, the complexity of the…
Language
Publication Number
20477
Publication Type
Tip&Trick
Source
The Column
Year
2019
Publication date
11.04.2019
Issue
4
Author
T. Hofe, M. Susewind
T&T GPC/SEC: Column Order in GPC/SEC
This instalment of Tips & Tricks will discuss the effect of column order on separation efficiency.
Language
Publication Number
20528
Publication Type
Tip&Trick
Source
The Column
Year
2022
Publication date
24.08.2022
Issue
4
Author
W. Radke
T&T GPC/SEC: Copolymer Analysis in GPC/SEC_1: Compositional analysis by GPC/SEC with multiple detection
GPC/SEC analysis reveals a multitude of molar mass information about unknown samples which are chemically simple or homogenous. However, results for copolymers may be compromised by the fact that…
Language
Publication Number
20339
Publication Type
Tip&Trick
Source
The Column
Year
2010
Publication date
08.06.2010
Issue
6
Author
P. Kilz
T&T GPC/SEC: Copolymer Analysis in GPC/SEC_2: Polymer Liquid Adsorption Chromatography
The CCD can be determined using several techniques including dual detection (see Tip&Trick copolymer analysis part 1). In this case GPC/SEC separation based on the size (hydrodynamic volume) is…
Language
Publication Number
20340
Publication Type
Tip&Trick
Source
The Column
Year
2010
Publication date
20.08.2010
Issue
8
Author
P. Kilz
T&T GPC/SEC: Cumulative Distributions and Cumulative Match Calibration
Typically calibration in GPC/SEC is accomplished using narrowly distributed standards of known molar masses yielding relative molar masses for the analytes. If narrowly distributed standards having…
Language
Publication Number
20512
Publication Type
Tip&Trick
Source
The Column
Year
2021
Publication date
19.01.2021
Issue
1
Author
D. Held, W. Radke
T&T GPC/SEC: Do's and Don'ts for Data Analysis_
GPC/SEC data processing requirements are fundamentally different from those of other liquild chromatographic techniques like for example HPLC. GPC/SEC users should follow some basics rules when the…
Language
Publication Number
20386
Publication Type
Tip&Trick
Source
The Column
Year
2013
Publication date
21.03.2013
Issue
2
Author
D. Held