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Publications

T&T GPC/SEC: How to validate a GPC/SEC system
Validation is not an easy task. It is an ongoing complex process that evaluates the entire, sample specific process, products, and analytical methods and consists of four qualification phases: DQ…
Language
Publication Number
20318
Publication Type
Tip&Trick
Source
The Column
Year
2010
Publication date
05.03.2010
Issue
3
Author
D. Held, C. Trimmer
T&T GPC/SEC: Influence of Sample Dispersity on the GPC/SEC Separation of Multi-Component Systems
This instalment of Tips & Tricks focuses on whether a complete separation can be achieved using GPC/SEC and, if so, under what conditions.
Language
Publication Number
20525
Publication Type
Tip&Trick
Source
The Column
Year
2021
Publication date
11.11.2021
Issue
11
Author
W. Radke, M. Lossa, J. Preis
T&T GPC/SEC: Inter-Detector Delay
Combinations of detectors are often used in gel permeation chromatography/size-exclusion chromatography (GPC/SEC) to measure absolute molar masses or chemical compositions as a function of elution…
Language
Publication Number
20441
Publication Type
Tip&Trick
Source
The Column
Year
2017
Publication date
10.08.2017
Issue
8
Author
D. Held, W. Radke
T&T GPC/SEC: Method Development_
The information and results that are available from detector combinations have been the subject of various Tips&Tricks over the years. Undiscussed up to know was the general impact of detector…
Language
Publication Number
20385
Publication Type
Tip&Trick
Source
The Column
Year
2012
Publication date
25.04.2012
Issue
12
Author
D. Held
T&T GPC/SEC: Method Optimization_
Many scientists consider the development of a GPC/SEC method easy, especially when compared to HPLC applications. This is true in the sense that normally only isocratic conditions are applied, so that…
Language
Publication Number
20387
Publication Type
Tip&Trick
Source
The Column
Year
2013
Publication date
24.05.2013
Issue
4
Author
D. Held
T&T GPC/SEC: Mobile Phase Considerations
There are many sources of advice on how to select the most appropriate stationary phase for sample analysis, but the mobile phase is not often discussed. The mobile phase is an essential and integral…
Language
Publication Number
20418
Publication Type
Tip&Trick
Source
The Column
Year
2014
Publication date
02.06.2014
Issue
4
Author
D. Held
T&T GPC/SEC: New and Old Approaches to Handle Unknown dn/dc in GPC/SEC-LS
The intensity of the light scattering signal depends on concentration, molar mass, and the specifi c refractive index increment, dn/dc, of the sample. dn/dc therefore usually needs to be known to…
Language
Publication Number
20439
Publication Type
Tip&Trick
Source
The Column
Year
2017
Publication date
26.10.2017
Issue
10
Author
W. Radke
T&T GPC/SEC: Particle Size and the Effect of Mixing Columns of Different Particle Size
Gel permeation chromatography/size-exclusion chromatography (GPC/SEC) columns are filled with porous particles differing in particle and pore sizes. Typical particle sizes in analytical GPC/SEC range…
Language
Publication Number
20514
Publication Type
Tip&Trick
Source
The Column
Year
2020
Publication date
20.08.2020
Issue
8
Author
D. Held, W. Radke
T&T GPC/SEC: Polydispersity - How Broad is Broad for Macromolecules?
The vast majority of macromolecules exhibit a molar mass distribution that is often described by the polydispersity index (PDI). This Tips & Tricks instalment offers practical advice to consider when…
Language
Publication Number
20442
Publication Type
Tip&Trick
Source
The Column
Year
2016
Publication date
22.12.2016
Issue
12
Author
D. Held, W. Radke
T&T GPC/SEC: Product Registration and REACH
Molecular mass is one of the central parameters required for product registration. Compared to low molar mass substances, the molar mass determination of macromolecular products is more diffi cult…
Language
Publication Number
20436
Publication Type
Tip&Trick
Source
The Column
Year
2017
Publication date
20.06.2017
Issue
6
Author
D. Held
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