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T&T GPC/SEC: Protein Analysis with Size-Exclusion Chromatography
Gel permeation chromatography/size-exclusion chromatography (GPC/SEC) is the standard method to separate samples by molecular size. In protein analysis, size-exclusion chromatography is either applied…
Language
Publication Number
20437
Publication Type
Tip&Trick
Source
The Column
Year
2017
Publication date
16.05.2017
Issue
4
Author
D. Held, T. Hofe
T&T GPC/SEC: Quantify and Get More Than Molar Mass Averages
Gel permeation chromatography/size-exclusion chromatography (GPC/SEC) is used to determine molar mass averages and the complete molar mass distribution with just one injection. This is possible…
Language
Publication Number
20435
Publication Type
Tip&Trick
Source
The Column
Year
2017
Publication date
23.02.2017
Issue
2
Author
D. Held
T&T GPC/SEC: Sample Recovery and More
Method validation often includes the measurement of sample recovery. There are easy ways to do this with gel permeation chromatography/size-exclusion chromatography (GPC/SEC) while taking a look at…
Language
Publication Number
20421
Publication Type
Tip&Trick
Source
The Column
Year
2014
Publication date
31.10.2014
Issue
10
Author
D. Held, P. Kilz
T&T GPC/SEC: Select the Right Columns for Your Molar Mass Range
Shoulders in gel permeation/size-exclusion chromatograms (GPC/SEC) can be a result of sample characteristics or down to the wrong choice of columns or column combinations. Proper selection helps to…
Language
Publication Number
20412
Publication Type
Tip&Trick
Source
The Column
Year
2013
Publication date
10.12.2013
Issue
12
Author
D. Held
T&T GPC/SEC: Separation Range and Resolution
Whereas the elution time in interaction chromatography can be widely varied by applying a suitable eluent composition or gradient slope, the separation in GPC/SEC is restricted by the interstitial and…
Language
Publication Number
20515
Publication Type
Tip&Trick
Source
The Column
Year
2021
Publication date
07.04.2021
Issue
4
Author
D. Held, W. Radke
T&T GPC/SEC: Silica Versus Polymer-Based Columns
The chemistry of samples analyzed using gel permeation chromatography/size-exclusion chromatography/gel filtration chromatography (GPC/SEC/GFC) is very diverse. Different chemistries of stationary…
Language
Publication Number
20509
Publication Type
Tip&Trick
Source
The Column
Year
2020
Publication date
03.02.2020
Issue
1
Author
D. Held, K. Oleschko
T&T GPC/SEC: Strategies to Save Solvent
Tetrahydrofuran (THF) - a widely used solvent in gel permeation chromatography/size-exclusion chromatography (GPC/SEC) - is set to be classifi ed as carcinogenic as proposed by the Committee for Risk…
Language
Publication Number
20431
Publication Type
Tip&Trick
Source
The Column
Year
2015
Publication date
07.08.2015
Issue
8
Author
J. Preis, D. Held
T&T GPC/SEC: System Peaks or Ghost Peaks in GPC/SEC
Extraneous peaks, unrelated to the solute to be characterized, are quite common in gel permeation chromatography/size-exclusion chromatography (GPC/SEC), especially when refractive index (RI)…
Language
Publication Number
20466
Publication Type
Tip&Trick
Source
The Column
Year
2019
Publication date
16.01.2019
Issue
2
Author
D. Held, F. Gores
T&T GPC/SEC: Systematic errors in GPC/SEC/GFC
GPC/SEC results and their validity are crucial in many applications, e.g. as part of QC/QA tests in pharmaceutical applications or for the registration of polymeric products or formulations with…
Language
Publication Number
20481
Publication Type
Tip&Trick
Source
The Column
Year
2019
Publication date
21.10.2019
Issue
10
Author
D. Held, P. Kilz
T&T GPC/SEC: Temperature effects in GPC/SEC
Temperature effects in GPC/SEC are very often misunderstood and discussed without keeping the specialties of GPC/SEC in mind. From a thermodynamically point of view the GPC/SEC separation mechanism is…
Language
Publication Number
20316
Publication Type
Tip&Trick
Source
The Column
Year
2009
Publication date
21.08.2009
Issue
8
Author
T. Hofe, D. Held