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T&T GPC/SEC: The Art of Analyzing High Molar Mass Samples
GPC/SEC is a method that can be used for a variety of samples with molar masses ranging from only a few hundred up to several million daltons. While handling of low molar mass samples is straight…
Language

Publication Number
20419
Publication Type
Tip&Trick
Source
The Column
Year
2014
Publication date
10.06.2014
Issue
6
Author
D. Held
T&T GPC/SEC: The importance of molar mass distributions
Why are molar mass distributions (MMD) important and what is the difference between a molar mass distribtution and a chromatogram?
Language

Publication Number
20254
Publication Type
Tip&Trick
Source
The Column
Year
2007
Publication date
31.08.2007
Issue
8
Author
D. Held
T&T GPC/SEC: Trouble Analyzing PEGs?
This instalment of Tips & Tricks will discuss experimental details that need to be considered when analyzing PEGs.
Language

Publication Number
20527
Publication Type
Tip&Trick
Source
The Column
Year
2022
Publication date
08.04.2022
Issue
4
Author
W. Radke, H. Berg
T&T GPC/SEC: UGPC? Making GPC/SEC faster
Ultra-HPLC (UHPLC) provides a new potential for method development and analysis. It is achieved by using sub-2 µm particle size column packing at increased linear velocities. The advantage of UHPLC…
Language

Publication Number
20324
Publication Type
Tip&Trick
Source
The Column
Year
2010
Publication date
10.04.2010
Issue
4
Author
D. Held, G. Reinhold, P. Kilz
T&T GPC/SEC: Understanding positive and negative detector signals
What are typical detectors in GPC/SEC? What are the differences between these detectors and their signals? Are negative detector signals possible and how can they be avoided? Application guide -…
Language

Publication Number
20288
Publication Type
Tip&Trick
Source
The Column
Year
2008
Publication date
04.12.2008
Issue
10
Author
D. Held, P. Kilz
T&T GPC/SEC: Upside Down - Inverse GPC for Pore Size Distributions
Gel permeation/size-exclusion chromatography (GPC/SEC) is the standard method for separating samples by molecular size and determining molar mass distributions. However, GPC/SEC instruments can also…
Language

Publication Number
20448
Publication Type
Tip&Trick
Source
The Column
Year
2016
Publication date
11.03.2016
Issue
2
Author
D. Held, P. Kilz
T&T GPC/SEC: UV-vis Detection
The most commonly applied detector in gel permeation chromatography/size-exclusion chromatography (GPC/SEC) is the differential refractive index detector, RI. How UV-vis detection, if applicable, adds…
Language

Publication Number
20464
Publication Type
Tip&Trick
Source
The Column
Year
2018
Publication date
15.08.2018
Issue
8
Author
D. Held
T&T GPC/SEC: What are the applications for (polymer) reference standards?
What types of reference standards are available? What are applications for (polymer) reference standards? Application guide - How are reference standards classified and characterized? Why do I need…
Language

Publication Number
20283
Publication Type
Tip&Trick
Source
The Column
Year
2008
Publication date
04.12.2008
Issue
8
Author
D. Held
T&T GPC/SEC: What are the differences between GPC, SEC, and GFC, and how do you get started with the technique?
Molar mass distributions, molar mass averages, and polydispersity can be determined by gel permeation chromatography (GPC), size-exclusion chromatography (SEC), and gel fi ltration chromatography…
Language

Publication Number
20468
Publication Type
Tip&Trick
Source
The Column
Year
2018
Publication date
14.12.2018
Issue
10
Author
D. Held
T&T GPC/SEC: What You Need to Know to Allow for Efficient GPC/SEC Troubleshooting
Although modern gel permeation chromatography/size-exclusion chromatography (GPC/SEC) instruments are generally very reliable, scientists sometimes encounter problems. This instalment of GPC/SEC Tips…
Language

Publication Number
20465
Publication Type
Tip&Trick
Source
The Column
Year
2018
Publication date
20.12.2018
Issue
12
Author
D. Held